97th DOG Annual Meeting 1999
P594
THE RETINA IMPLANT-PROJECT (EPI RET): TOPOHISTOLOGICAL 3-D RECONSTRUCTION OF THE DISTRIBUTION OF RETINAL LAYER THICKNESS IN RABBITS
F. P. Bollmann, S. Taneri, C. E. Uhlig, S. Kupich, H. Gerding
The aim of this study was to develop a topohistological layer-depending standard of the complete rabbit retina. The found data are meant to make the evaluation of minimal lesions, apoptosis and neuroprotection after surgical retinal manipulations possible.
Materials and methods: The experiments were performed on 30 eyes of albino rabbits destined for slaughtering (breed: zicker; age: app. 14 weeks; weight: app. 3.2 kg). The eyes were fixed in phosphate-buffered formaldehyde immediately after the killing. In order to get data from all areas of the retina, longitudinal sections were placed at 0º (horizontal position directly below the optic nerve), at 45º, 90º (vertical cut through the optic nerve) and 135º. 5µm thick sections of the embedded tissue blocks were cut and stained with hematoxylin and eosin. The histological study slides were microphotographed. By means of the slides the different retinal layers were measured at 17 preselected locations of the optic fundus, taking all enlargement factors into account.
Results: A topohistological standard of the thickness of retinal layers could be developed which can be used for the evaluation of minimal lesions, thus making measurement and evaluation of the retinal affection caused by fixation techniques in long-term studies.
Sponsored by the BMBF (German National Ministery of Education and Research) Co-operation within the EPI RET-Consortium (01 IN 501)
Dept. Of Ophthalmology, Univ. of Münster, Domagkstr. 15, 48129 Münster, Germany
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