97th DOG Annual Meeting 1999
V689
IN VITRO INHIBITION OF BOVINE AND HUMAN LENS EPITHELIAL CELL ADHESION WITH A CYCLO-RGD-PEPTIDE
C. Fritzsche, P. Rieck, H. Baatz
Purpose: Studies on the inhibition of posterior capsule opacification using cytotoxical substances have shown significant side-effects on other ocular tissues. First investigations on the inhibition of adhesion and migration of lens epithelial cells (LEC) by RGD-peptides via a competitive block of integrin receptors were promising.
Methods: Bovine and human LEC were grown in culture. Cells were seeded onto uncoated culture dishes to investigate the inhibition of adhesion after different times of incubation (1 hour, 5 days resp.) and different concentrations of the RGD-peptide (10-3 M, 10-4 M resp.). The number of attached cells was counted after 1, 48, 72, 120,168 hours. Furthermore, we investigated the potency of the peptide to detach confluent monolayers from the bottom of the culture dish resp. from bovine anterior lens capsules. Both experimental groups were compared to control cultures (pure medium) and a control-peptide.
Results: The inhibition of adhesion of bovine LEC was 100% when the RGD-peptide was incubated for 5 days in a concentration of 10-4 M. After one hour of incubation the rates of inhibition were 46 % (10-4 M) and 56 % (10-3 M). The inhibition of adhesion of human LEC incubating the peptide for 5 days (10-4 M) was 86 %. Adherent LEC detached from the bottom of the dish after about 33 minutes (RGD-peptide 10-4 M). There was no detachment of confluent LEC from excised anterior capsules with a concentration of 10-3 M.
Conclusions: The results of our study indicate the potency of this RGD-peptide to inhibit LEC-adhesion in vitro. However, there was only a minor effect on LEC adhering on capsules.
Department of Ophthalmology, Charité, Campus Virchow-Klinikum, Augustenburger Platz 1, D-13353 Berlin
supported by DFG Ri 568/3-2
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