P 270
Genistein induced growth control in microvascular endothelial cells is not alterated by TaurinR. Krott, N. Kociok, M. Lüke, C. Lüke, P. Esser, K. U. Bartz-Schmidt
Purpose: Concentration-dependent growth control can be achieved in human microvascular endothelial cells (HMVEC) can be achieved in vitro and in vivo with by Genistein, a known protein tyrosine kinase inhibitor. Therefore, it genistein is an interesting agent for treating ocular neovascularization. Due to its neurotoxicity we added the neuroprotective amino acid taurine to the assay to elucidate, if genistein still has its antiproliferative effects on HMVEC.
Methods: HMVEC (passage 3-5) from adult dermis (TEBU) were cultured at concentrations of 104 cells/well in microtiter plates (96 wells) in a final volume of 100 µl/well culture medium 131 for 24 hours. Then an exchange of the culture medium was performed with media including genistein (at concentrations: 12.5µg/ml, 25µg/ml, 50µg/ml, 100µg/ml) and VEGF (10ng/ml) and incubated for 24 hours. As control served MmediumMedium 131 including DMSO (10µl/ml) and VEGF served as controls. The same assay was repeated with medium additionally containing taurine (375.3µg/ml). The overall activity of mitochondrial dehydrogenases in the proliferating cells was measured by the WST-1 test (Roche).
Results: Genistein (at 12.5µg/ml, p=0.0051, paired t-test) induced a significant (at 12.5µg/ml, p=0.0051, paired t-test) concen-tration dependent growth control of in HMVEC and blocked completely VEGF induced cell proliferation (p=0.0005, unpaired t-test). with and without tTaurine showed no measurable effekt on the Genistein induced growth control.
Con-clusions: Taurine does seems not seem to interfere alterate with the antiproliferative effects of genistein on in HMVEC in vitro. This These results might indicate an interesting new treatment approach for ocular neovascularization.
Dep. of Ophthalmology, University of Cologne; D-50924 Cologne
supported in part by the Retino-Vit foundation