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Impressum
Adhesion of Lens-epithelium-cells on different IOL-Materials: influence of a new surface-modification
1Bunse A., 2Menz D.-H., 3Hoerauf H.
1University-eye-hospital Regensburg; 2Pharm Pur GmbH Augsburg; 3University-eye-hospital Lübeck
Introduction: The adhesion of lens-epitheliun-cells (LEC) on intraocular
lenses (IOL) is crucial for the development of posterior capsule opacification
(PCO). Aim of this study is to test the adhesion criteria of LEC on different
IOL-materials with a surface-coating by Dynasilan®. Methods: 54 IOLs
(18 PMMA, 18 Silicone, 18 Acrylic) were tested. 9 IOLs of each material
were coated with Dynasilan®. Rabbit LEC in passage 3 were cultivated
on each IOL for 72 h (10³ cells). After fixation the cells were counted.
LEC on 3 IOLs of each material were tested for the adhesion molecule vinculin
by antibody staining.
Results: Dynasilan® -coating reduced the adherent LEC on PMMA-IOL
by 72.6% and on Silicone-IOL by 32%. Vinculin-antibody-staining on these
these cells showed a reduced fluorescence. The Acrylic-IOL was opaque
after the Dynasilan® -coating. The number of LEC was increased by
55%.
Conclusion: In PMMA- and Silicone-IOL the number of adherent LEC
was significantly reduced by Dynasilan® -coating. These in vitro results
suggest a reduced PCO-rate. In vivo testing has to be performed to varify
these results. These preliminary results show that Acrylic-IOL are not
appropriate for a Dynasilan® -coating.