Anmeldung zur Tagung
Registration
Grußwort
Invitation
Themen
Topics
Allgemeiner Ablauf
General overview
Wissenschaftliches Programm
Scientific program
Kurse
Courses
Symposien
Symposiums
Frühstück mit Spezialisten
Breakfast with specialists
Arzthelferinnen-Fortbildung
Rahmenprogramm
Social program
DOG Information
DOG Information
Allgemeine Informationen
General Information
Autorenindex
Index of Authors
Ausstellerliste
Exhibitors
Sponsoren
Sponsors
Teilnahmegebühren
Registration fees
Impressum
HLA class I antigen expression correlates with histologic cell type in uveal melanoma
1,2Dithmar S., 2Crowder J., 3Jager M. J., 4Vigniswaran N., 2Grossniklaus H. E.
1Dep. of Ophthalmology, University of Heidelberg, Germany, 2Dep. of Ophthalmology, Emory University School of Medicine, Atlanta, GA, 3Dep. of Ophthalmology, Leiden, Holland, 4Dep. of Oral Pathology, University of Alabama at Birmingham, Birmingham, AL
Purpose: The prognosis of uveal melanoma is correlated with its
histologic cell type. The epithelioid cell type is associated with a higher
metastatic rate than the spindle-cell type. The Human-Leucocyte-Antigen-Class
I (HLA-I)- expression of the melanoma also correlates with prognosis.
In this study, we analyzed HLA-I antigen expression by uveal melanoma
to determine whether a relationship exists between antigenic expression
and melanoma cell type.
Methods: Formalin-fixed, paraffin-embedded spindle cell type (n=11)
and epithelioid cell type (n=11) uveal melanomas were immunostained with
the HC10 antibody (1:80) for HLA-I antigen expression with appropriate
positive and negative controls. Staining intensity was graded as 0 for
no staining, + for 1-25% of cells staining, ++ for 26-50% of cells staining,
+++ for 51-75% of cells staining and ++++ for 76-100% of cells staining.
Among the spindle cell type melanomas, 2 of 11 (18%) stained with
Results: HC10 antibodies. The staining intensity was less than
25% of the cells in these two melanomas. Among the epithelioid cell type
melanomas, 9 of 11 (82%) stained with HC10. The staining intensity was
more than 25% of the cells in 5 of these 9 melanomas.
Conclusions: It has been unknown why spindle and epithelioid cell
type uveal melanomas have different prognosis. Human uveal melanoma cell
lines with low HLA-I expression are susceptible to NK-cell mediated lysis
in vitro and in murine studies. The prognostic more favorable spindle
cell type melanoma expresses less HLA-I than the epithelioid cell type
melanoma. These results stress the role of NK cells in the rejection of
uveal melanoma.
Universitäts-Augenklinik Heidelberg, INF 400, 69120 Heidelberg Supported
by Gertrud Kusen Foundation, Germany (DI 98/99), RPB Inc. and NIH EY06360