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Impressum
Effect of autologues serum on wound-healing of non-infectious ulcers und expression of growth factor receptors in the cornea
Ebner S., You L., Völcker H. E., Kruse F. E.
Department of Ophthalmology, University of Heidelberg, INF 400, 69120 Heidelberg
Serum contains various factors, which influence cellular proliferation,
differentiation and wound-healing. We investigated the effect of concentrated,
autologues serum on wound-healing of non-infectious corneal ulcers. For
elucidation of the mechanisms the expression of growth factor receptors
was investigated in cell culture.
Methods: In vivo epithelial wound-healing after treatment with
autologues serum (100 %) was investigated in 37 patients with non healing
ulcers due to keratitis herpetica, rheumatic ulcers, bacteria keratitis
or pemphigoid. Invitro primary cultures of human keratozytes were used.
Cells were incubated in presence or absence of serum or 1 ng/ml TGF-ß1
for 15 hours and followed by RNA isolation. Expression of growth factor
receptors (ActR-I and II, BMPR-IA and II, TrkA, B, and C) were examined
by Dot- Blots.
Results: Treatment with serum (mean 13 x /day) lead to complete
epithelial healing in 25 of 37 patients within 11 ± 6 days. In
6 patients a healing process was detected after 10 ± 4,5 days,
in 6 patients no woundhealing was induced by serum. Serum induces a significant
increase of RNA for different growth factor receptors. Of the nerve growth
factors Trk A, B and C were upregulated. Also an increase of RNA for receptors
of the TGF-ß family was observed (Activinreceptors: ActR-I and II,
and BMPreceptors BMPR-I und II).
Discusion: Our results confirm, that serum can be used as treatment
for non-infectious corneal ulcers. The mechanism seems to be mediated
by factors present in the serum, such as growth factors and fibronectin.
Furthermore it seems that serum makes corneal cells more sensitive for
these factors by increasing the number of receptors on the cell surface.
DFG (Kr 993/12-1)