Anmeldung zur Tagung
Registration
Grußwort
Invitation
Themen
Topics
Allgemeiner Ablauf
General overview
Wissenschaftliches Programm
Scientific program
Kurse
Courses
Symposien
Symposiums
Frühstück mit Spezialisten
Breakfast with specialists
Arzthelferinnen-Fortbildung
Rahmenprogramm
Social program
DOG Information
DOG Information
Allgemeine Informationen
General Information
Autorenindex
Index of Authors
Ausstellerliste
Exhibitors
Sponsoren
Sponsors
Teilnahmegebühren
Registration fees
Impressum
The presence of important regulators of apoptosis in human tenon fibroblasts.
Esser J. M., Welsandt G., Luther T., Jordan J. F., Esser P., Krieglstein G. K.
University of Cologne, Germany, Josef Stelzmann Str. 9, 50931 Köln
Objective: Programmed cell death or apoptosis is a process, whereby
developmental or environmental stimuli cause cell death, through a natural,
energy-dependent process. Several important regulators of this process
have been identified. Among them the tumor suppressor protein p53, Bcl-2
and Bax protein have been attributed a major role in the complex signaling
pathway of apoptosis induction or inhibition. Here, we sought to investigate
the presence of these proteins in human tenon fibroblasts, which are thought
to play a major role in scar formation after glaucoma surgery. Furthermore
we investigated the influence of topical glaucoma medication (Latanoprost,
Timolol) on these proteins.
Methods: RT-PCR: Total RNA was prepared by guanidium/phenol extraction
from cultured human tenon fibroblasts of passage P2. The presence of p53,
Bcl-2 and Bax was investigated by RT-PCR using specific PCR primers. Furthermore
the expression of p53, Bcl-2 and Bax was investigated in cultured human
tenon fibroblasts by immunoblot analysis and Fluocytometry.
Results: Strong, specific bands for the chosen primer combinations
could be detected by RT-PCR in P2 cells for all proteins. Fluocytometry
revealed, that all the investigated proteins are present in human tenon
fibroblast cells on the protein level. More interestingly, the glaucoma
medication led to a significant rise of expression for all proteins. In
contrast, only p53 could be detected by immunoblot analysis after treating
the cells for 72hours with camptothecin
Conclusion: The presence of the important regulators of apoptosis
p53, Bcl-2 and Bax in human tenon fibroblasts has implications for future
studies of antiproliferative and proapoptotic therapeutic strategies in
the inhibition of scar formation after glaucoma surgery. Therefore, the
presence of all the investigated proteins in human tenon fibroblasts may
become useful in the engineering of antiproliferative agents that induce
apoptosis in proliferating cells while sparing normal cells. Especially
the increased expression of the proteins after topical glaucoma medication
leads to new perspectives in the field of scar formation after glaucoma
surgery.