1Hoerauf H., 1,2Kienast A., 3Menz D.-H., 1Laqua H., 2Solbach W., 2Kämmerer R.

1Klinik für Augenheilkunde, Universitätsklinikum Lübeck; 2Institut für Med. Mikrobiologie und Hygiene, Universitätsklinikum Lübeck; 3Pharm Pur GmbH, Augsburg

Introduction: Dynasilan®, a fluoroalkylsilan, is able to block active surface molecules on intraocular lenses (IOLs). The purpose of the study was to investigate the influence of this new surface modification on the adherence of bacteria (Staphylococcus epidermidis, Propionibacterium acnes) which are commonly found in postoperative endophthalmitis.
Materials and methods: 42 Dynasilan®-modified and 42 unmodified IOLs (PMMA, silicone and acryl) were incubated in BHI-broth (10⁸ CFU/ml) for 24h with S. epidermidis (aerob) or for 1h with P. acnes (anaerob). Subsequently the adherent bacteria were resuspended using ultrasonication at 35kHz for 3x45sec and dilution series were done. After incubation at 37°C for 24h and 3d respectively the colonies were counted.
Results: On unmodified IOL's incubated with S. epidermidis the average number of bacteria was: silicone 3,60x10⁷/ml, acryl 2,31x10⁷/ml and PMMA 1,38x10⁷/ml. On modified IOL's the number was reduced to: acryl 1,98x10⁷/ml, silicone 1,09x10⁷/ml and PMMA 1,04x10⁷/ml. Incubated with P. acnes the number of bacteria on unmodified IOLs was: PMMA 7,98x10⁴/ml, acryl 6,07x10⁴/ml and silicone 4,75x10⁴/ml. On modifiied IOLs the average number of bacteria was reduced to: silicone 2,66x10⁴/ml, PMMA 1,78x10⁴/ml and acryl 1,22x10⁴/ml.
Conclusion: The presented in vitro-results indicate that the adherence of endophthalmitis causing bacteria on different IOL-types was reduced markedly by Dynasilan®- surface modification. For S. epidermidis the strongest effect was seen on silicone-lenses, for P. acnes on PMMAlenses. Further studies regarding biocompatibility and stability are currently performed.

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