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Impressum
Effects of "advanced glycation endproducts" on choroidal endothelial cells and retinal pigmented epithelial cells.
Hoffmann S., Eichler W., Friedrichs U., Rosenthal A., Tratz C., Wiedemann P.
Universitätsaugenklinik Leipzig
Purpose: Choroidal Neovascularization (CNV) is a complication
of exudative, age-related macular degeneration (AMD) leading to irreversible
blindness in the elderly. Advanced glycation endproducts (AGEs) are considered
to be involved in the pathophysiology of exsudative AMD. In this study,
we are evaluating the effects of AGEs on the proliferation and migration
of choroidal endothelial cells (CECs) and retinal pigmented epithelial
cells (RPEs). In addition, AGE effects on the secretion of matrix metalloproteinases
(MMPs) by CECs and VEGF by RPEs were investigated.
Methods: Bovine CECs and RPEs were stimulated for three days with
AGEs. Cell proliferation was estimated by use of an BrdU assay and cell
migration by use a modified Boyden chamber. The conditioned medium of
AGE stimulated CECs was used for the Zymography and RPE conditioned medium
for the VEGF-ELISA.
Results: CECs stimulated in a dose dependent manner (10 - 100 µg/ml)
the proliferation of CECs. No effect on RPE proliferation was seen. Contrary,
the RPE migration was increased by AGEs but no effect on CEC migration
was seen. In addition, AGEs increased the secretion of MMP-2 by CECs and
the secretion of VEGF by RPEs.
Conclusion: AGEs modulate the proliferation and MMP-2 production
of CECs. In addition, they enhance the VEGF secretion and migration of
RPEs. Therefore, AGEs may be of importance for the initiation and progression
of exudative AMD.