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Impressum
Corneoscleral complex excision in obtaining donor corneas - a risk for the sterility of the organculture?
Jaroszewski, J., Jaeckel, C., Rieck, P.
Universitätsaugenklinik Charité, Hornhautbank Berlin, Campus Virchow- Klinikum, Augustenburger Platz 1, 13353 Berlin
Background:The excision of the isolated corneoscleral complex
is an alternative to the excision of the entire globe in obtaining donor
corneas. We are performing both methods and have compared the rate of
infection of the medium in organcultured corneas.
Material and method: The excision of the donor's eye is performed
under aseptic conditions with a non-touch technique. Prior to the sterile
preparation in the laminar air flow in the eye bank it is disinfected
in an iodine solution. Before the isolated corneoscleral complex is excised
an iodine disinfection of the skin and conjunctiva is performed in the
donor. All corneas are stored in organculture under the same conditions.
After 7 days and at the point of transferring the cornea into dextran
containing medium sterility test are performed.
Results: So far 200 corneas obtained by globe (group A) an 54 obtained
by isolates corneoscleral complex excision (group B) could be evaluated
for this study. 11 corneas (5,5%) of group A and 1 cornea (1,8%) of group
B became non-sterile.
Conclusion: The method of taking just the corneoscleral complex
from the donor is not associated with a higher risk of microbial contamination
in organculturing.