Knorrn M., Eichhorst A., Hanschke R., Duncker G. I. W., Wilhelm F.

Augenklinik der Martin-Luther-Universität Halle/Wittenberg, Magdeburger Str. 8, 06097 Halle/Saale

Purpose: The use of new methods of operation in cataract surgery require comparing investigations with conventional procedures. The objective of this study was the comparison of the corneal endothelium in relation to cell aggregation and histology of the single cell in the incision area and in the central cornea immediately after the insertion of diverse surgical procedures.
Materials and methods: Respectively 8 freshly enucleated pig eyes (1 hour post mortem) were prepared for every method of operation (ultrasound phacoemulsification with Sleeve, bimanual technique of ultrasound phacoemulsification without Sleeve, Erbium laser phacoemulsification) corresponding to the principle of cataract surgery. One or two inlets were laid into anterior chamber for the instruments. Capsulorhexis was carried out with canula. The operating time of the used energy for lens fragmentation always amounted to 1 minute. Uninfluenced corneal material was examined for comparison. The corneal disks were taken with the help of the trephine (diameter 8mm) for scanning-electronmicroscopic examination after preparation.
Results: The examinations have shown that the damage of endothelium cells was lowest in the area of incision by ultrasound phacoemulsification with Sleeve. Evident thermal damages of cells and cell aggregation were found in this area by ultrasound phacoemulsification without Sleeve and by Erbium laser phacoemulsification. In the centre of cornea alterations after all three operation methods were marked less. Clear differences have not occurred between them.

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