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Impressum
Trypan blue - effect on lens epithelial cells in vitro on human capsular bags
Liekfeld A., van Dooren B., Rieck P., Beekhuis W. H., Hartmann C.
Dept. of Ophthalmology, Charité, Campus Virchow-Klinikum, Augustenburger Platz 1, D-13353 Berlin
Background: Trypan blue (Vision Blue®) is used in cataract
surgery for lens capsule dying to make capsulorhexis easier. Some studies
have shown a toxic effect of trypan blue on special cells as corneal endothelial
cells. To examine a similar effect of trypan blue on lens epithelial cells
we conducted the following investigation.
Material and Methods: Sham cataract surgery, including anterior
capsulorhexis, nucleus hydroexpression, and aspiration of lens fibers,
was performed on 22 donor eyes (11 pairs) after removal of the cornea.
The capsular bag was dissected free, pinned flat on a culture dish and
covered with medium. One capsular bag of a donor eye pair was incubated
with trypan blue (concentration of 0.1% to 0.5%) for 24 hours, the second
capsular bag was used as a control. It was documented how long it lasted
until the posterior capsule was totally covered by a confluent monolayer
of lens epithelial cells. In some cases vital staining was performed for
quality assessment of the lens epithelial cells.
Results: Posterior capsule was totally covered by a confluent monolayer
of lens epithelial cells at 12.3±1.5/ 14±3.5/ 15.7±2.5
days after incubation with 0.1% (3 eyes)/ 0.25% (5 eyes)/ 0.5% (3 eyes)
trypan blue and at 11.3±3.1/ 8.6±1.5/ 8.7±3.1 days
in the controls. The differences were statistically significant for the
two higher concentrations. In vital staining there were more avital cells
after incubation with trypan blue.
Conclusion: Only for concentrations and durations of incubation
that are not relevant in clinical use of trypan blue we could show a toxic
and potentially inhibitory effect on lens epithelial cells in this in
vitro study on human capsular bags. So the clinical use of Vision Blue®
does not seem to have an effect on posterior capsule opacification after
cataract surgery.