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Impressum
Inhibition of proliferation of human Tenon's capsule fibroblasts in vitro with 1,25(OH)2 vitamin D3 analogues of 1,25(OH)2 vitamin D3 and 9-cis-retinoic acid
Rieger R., Rieck P.
Department of Ophthalmology, Charité Campus Virchow, Humboldt University, Berlin/Germany
Objective: The role of 1,25(OH)2 vitamin D3 and 9-cis-retinoic
acid in the regulation of cell proliferation has been previously described
for various cell lines. Treatment with new 1,25(OH)2 analogs and 9-cis-retinoic
acid has recently been described to irreversibly inhibit proliferation
of human prostate cancer cells. In our experiments we attempted to reproduce
similar results in Tenon's capsule fibroblast cell cultures in order to
find possible evidence for future clinical use to reduce scarring in glaucoma
surgery.
Methods: Tenon's capsule fibroblasts cultivated from 3 patients
aged 8 - 59 years were treated with 1,25(OH)2 vitamin D3, new analogs
of 1,25(OH)2 vitamin D3 and 9-cis-retinoic acid in concentrations ranging
from 10-6 to 10-10 M over a timeperiod of 92 h. After treatment the number
of fibroblasts was assessed via measurement in a coulter-counter and compared
with the untreated control group. Cell morphology was analized using electronmicroscopy.
Results: Treatment with 9-cis-retinoic acid at a concentration
of 10-6M led to a reduction in cell proliferation of 10% compared to the
untreated control group (p<0.0001). At a concentration of 10-10M retinoic
acid produced an increase in cell number of 10% (p=0.01). 1,25(OH)2 Vitamin
D3 caused a reduction in cell number of 10% (p<0.0001), the analogues
of 1,25(OH)2 Vitamin D3 had a similar effect reducing cell numbers by
10% (p<0.0001).
Conclusion: Treatment with 9-cis Retinoic acid and 1,25(OH)2 Vitamin
D3 and analogs of 1,25(OH)2 Vitamin D3 caused a statistically significant
reduction in proliferation of human Tenon's capsule fibroblasts. These
results could indicate a first step to a possible future use of these
drugs to reduce scarring after glaucoma surgery.