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Impressum
Photocoagulation of human retinal pigment epithelial cells results in an increased production of pigment epithelium-derived factor (PEDF)
1Schacke W., 1Hattenbach L.-O., 2Beck K. F., 1Koch F., 2Pfeilschifter J.
1Klinik für Augenheilkunde, 2Pharmazentrum Frankfurt, Johann-Wolfgang- Goethe-Universität, Frankfurt/Main
Objective: Photocoagulation constitutes an important method for
the treatment of various neovascular diseases of the posterior segment.
Although the exact mechanisms remain unclear, an increased production
of anti-angiogenic substances in laser-treated eyes can be observed. With
this study we sought to investigate, whether photocoagulation stimulates
the production of PEDF (pigment epithelium-derived factor) by human retinal
pigment epithelial cells in vitro.
Methods: Cultures of human retinal pigment epithel cells were photocoagulated
by using a 594 nm diode laser. The expression of PEDFmRNA was investigated
by RT-PCR, whereas PEDF-protein levels were determined by western blot
analysis.
Results: After laser-photocoagulation a significant increase in
PEDFmRNA, as well as PEDF-protein synthesis was observed.
Conclusion: The current findings indicate that PEDF may play a
critical role in the remission of intraocular neovascularization after
photocoagulation. An understanding of the mechanisms involved in retinal
or choroidal neovascularization may assist in the design of new treatment
strategies.
Supported by : August Scheidel Stiftung