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Impressum
Effect of Prostaglandin F2a on Proliferation and Collagen I Expression in human Keratocytes in vitro
Schmidt S., Lechner S., Rieck P. W.
Department of Ophthalmology, Charité / Campus Virchow, Humboldt University, Berlin, Germany.
Purpose: Latanoprost,a phenyl- substituted synthetic analogue
of prostaglandin (PG) F2a- isopropylester, has been shown to be
an effective intraocular pressure lowering agent in normal, ocular hypertensive
and glaucomatous eyes. The pressure lowering effect of Latanoprost eye
drops may be caused by a reduction of collagen I expression within the
uveoscleral outflow pathway. The modulation of cell proliferation and
collagen I synthesis in various tissues are major effects of PGF2a.
However, up to now it remains unknown whether PGF2a modulates also
the proliferation and collagen I expression in human keratocytes.
Methods: Human keratocytes (HK) were cultured according to established
protocols. Experiments were performed with cells of the 1. and 2. passage
under serum reduced conditions. 104 cells /well were seeded and cells
were then incubated with different (5 x10-5 - 5 x10-3 mg/ml) concentrations
of PGF2a (Sigma Ind.).The number of cells was determined after
24, 72 and 120 hours. The modulation of collagen I expression was tested
in PGF2a treated cultures by immunofluorecences cytochemistry using
fetal mouse anti - collagen I antibodies.
Results: Depending on the concentration PGF2a induced a
significant stimulation of HK proliferation(more then a 2 fold increase
in cell number at the highest concentration of PGF2a after 120
hours).The collagen I expression was reduced in PGF2a treated cell
cultures in comparison to unsupplemented control cultures.
Conclusion: For the first time the present study demonstrates the
effect of PGF2a on proliferation and collagen I expression in HK.
It appears interesting that the number of cells increases but the expression
of collagen decreases in PGF2a treated cell cultures.The reduction
of collagen could be an answer to the thinning of the cornea after prolonged
use of Latanoprost eye drops described in other studies before.