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Impressum
Analysis of chromosomal breaks in cultured human keratocytes after excimer-laser photoablation
Sherif Z., Neitzel H., Hartmann C., Pleyer U.
Department of Ophthalmology, Department of Human Genetics, Charité Campus Virchow, Humboldt University, Berlin/Germany
Purpose: To evaluate the rate of chromosomal breaks in cultured
human keratocytes after excimer-laser photoablation.
Material and methods: Three pairs of human corneas which were not
suitable for therapeutic uses were investigated. One of each pair underwent
excimer-laser photoablation including the epithelium, bowman´s layer
and anterior stroma (193 nm, 180 mJ/cm², 4Hz). The second cornea
of each pair served as control. After isolation, the keratocytes were
cultured using Amniomax®. Keratocytes were stained with giemsa and
GTG and were analysed for chromosomal breaks performing ISCN (5 analysis
per culture).
Results: The rate of chromosomal breaks in keratocytes of corneas
which underwent photoablation was 0.16/cell (SD=0.06) compared to 0.12/cell
(0.04) in keratocytes from corneas which served as controls (p>0.05).
Conclusion: There was no statistically significant difference in
the rate of chromosomal breaks in keratocytes from human corneas which
underwent excimer-laser ablation compared to keratocytes from corneas
which served as controls.