Anmeldung zur Tagung
Registration
Grußwort
Invitation
Themen
Topics
Allgemeiner Ablauf
General overview
Wissenschaftliches Programm
Scientific program
Kurse
Courses
Symposien
Symposiums
Frühstück mit Spezialisten
Breakfast with specialists
Arzthelferinnen-Fortbildung
Rahmenprogramm
Social program
DOG Information
DOG Information
Allgemeine Informationen
General Information
Autorenindex
Index of Authors
Ausstellerliste
Exhibitors
Sponsoren
Sponsors
Teilnahmegebühren
Registration fees
Impressum
Toxic effect of soluble and crystalline triamcinolone acetonide on RPE and choroid
Spandau U., Jonas J. B.
Universitätsaugenklinik Mannheim, Fakultät für Klinische Medizin Mannheim der Universität Heidelberg
Background: In recent pilot studies, triamcinolone acetonide has
been used as treatment of intraocular neovascular, edematous and inflammatory
diseases as ARMD and diabetic retinopathy. The purpose of the present
study was to investigate the toxic effect of soluble and of crystalline
triamcinolone acetonide (TA) on the eye.
Material and Methods: Primary cultured human RPE cells were incubated
with soluble and crystalline TA in concentrations of 2mg/ml and 4mg/ml
for 10 days. The number of proliferating cells was counted and the morphology
of RPE cells was evaluated. BrdU-ELISA was used to measure the proliferation
rate of RPE cells. Porcine eyes were dissected and incubated with soluble
and crystalline TA.
Results: After 10d incubation with crystalline TA, the number of
human RPE cells decreased moderately. Human RPE cells incubated with soluble
TA, showed after 3d a marked decline in the number of cells. The number
of not incubated human RPE cells increased continuously. Morphologically,
the RPE cells showed an increase in cell volume. Human RPE cells incubated
with crystalline TA showed after 3d in BrdU ELISA an increased proliferation.
After incubation with soluble TA, human RPE cells showed a strong decrease
in proliferation. After incubation for 7 days with crystalline TA, dissected
porcine eyes showed no morphological changes. After incubation with soluble
TA, RPE and choroidal tissue showed significant atrophy and hyperplasia.
Conclusion: The results indicate that soluble, but not crystalline,
triamcinolone acetonide in a concentration of 2 mg/ml and 4 mg/ml is toxic
to ocular structures such as RPE and choroid.