Anmeldung zur Tagung
Registration
Grußwort
Invitation
Themen
Topics
Allgemeiner Ablauf
General overview
Wissenschaftliches Programm
Scientific program
Kurse
Courses
Symposien
Symposiums
Frühstück mit Spezialisten
Breakfast with specialists
Arzthelferinnen-Fortbildung
Rahmenprogramm
Social program
DOG Information
DOG Information
Allgemeine Informationen
General Information
Autorenindex
Index of Authors
Ausstellerliste
Exhibitors
Sponsoren
Sponsors
Teilnahmegebühren
Registration fees
Impressum
Kinetics of C-erbB oncoprotein and CD44 isoform expression in the IRBP induced Experimental Autoimmune Uveitis (EAU)
1Stiemer R. H., 1Fischer-Lamprecht C., 2Wiggert B., 3Zöller M., 4Günthert U., 1Zierhut M.
1Dept. of Ophthalmology, University of Tübingen, Germany; 2National Eye Institute, Bethesda, MD, USA; 3German Cancer Research Center, Heidelberg; 4Basel Institute for Immunology, Switzerland
Purpose: Overexpression of oncogen receptor c-erbB-2 in tumors
and functional involvement of CD44 isoforms in inflammation, autoimmune
diseases and tumors have initiated our analyses concerning their expression
in the IRBP induced EAU.
Methods: B10.A mice were immunized subcutaneously with 50µg
of IRBP. Immunohistochemical analysis of the eyes was achieved from animals
sacrificed on day 10, 18, 24 and 28 (n=5 for each time point). Applied
mAbs were directed against c-erbB-2, standard CD44 (CD44s, two different
epitopes), splicing variants v7 (exhibiting inhibitory functions in different
experimentally induced autoimmune diseases) and v10. Staining was performed
using the alkaline-phosphatase method with Neufuchsin as chromogen. Control
staining was achieved on eyes omitting IRBP in the immunization protocol
and on murine tongue sections.
Results: C-erbB oncogen receptors 2 were expressed in the ciliary
body, RPE, around the optic nerve and venules localized in different retinal
cell layers. Aquivalent results were observed for CD44s and CD44v7. More
staining was observed in retinal destruction which continously was reduced
until reaching uninflamed tissue. No expression was observed for CD44v10.
While CD44s and CD44v7 peaked on day 18 and decreased by day 28, c-erbB-2
was maximally expressed on day 24 after immunization. Control eyes exhibited
significantly reduced staining.
Conclusion: The distinct expression of CD44v7 in EAU may be important
in the interaction between Th1 cells and macrophages and their survival
as it has been analysed for different murine Th1 dependent autoimmune
diseases. Low level expression of c-erbB-2 and CD44 in control eyes indicates
that these proteins are not newly synthesized but are stronger expressed
under pathological conditions. Functional association of CD44 isoforms
and c-erbB oncogen-receptors and their role in cell biology and immunopathology
of uveitis will be discussed.
Support: ƒortuene grant 114, DFG-grant ZI 350/13.