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Impressum
Expression of PDGF-receptor type beta in human tenon's capsule fibroblasts after stimulation with cytokines in vitro
Von Eicken J., Denk P. O.
University Eye Clinic Tübingen, Dpt. I
Previously we showed, that PDGF isoforms are the most potent stimulators
of proliferation of human tenon's capsule fibroblasts (HTF) in vitro.
The aim of the present study was to investigate, whether treatment of
HTF with different cytokines modifies the expression of the corresponding
cell surface receptor PDGF receptor type beta (PDGFR-ß). Second
passage HTF were stimulated for six days with the following cytokines
at respective concentrations (PDGF-AA 0,01 - 100 ng/ml, PDGF-AB 0,01 -
100 ng/ml, PDGF-BB 0,01 - 100 ng/ml, EGF 0,01 - 100 ng/ml and IL-1 ß
0,001 - 10 ng/ml). The expression of PDGFR-ß was detected using
polyclonal antibodies in the Western Blot and analysed semiquantitatively
with densitometric techniques. The analysis of the immunoblot results
demonstrated a significant reduction of expression of PDGFR-ß after
stimulation with 100 ng/ml PDGF-AB and 100 ng/ml PDGF-BB. In contrast,
the remaining cytokines did not alter expression of PDGFR-ß even
at high concentration levels. Our results suggest, that high concentrations
of PDGF-AB and PDGF-BB lead to a reduced expression of PDGFR-ß during
wound healing, thus modifying cell proliferation in the wound region.