Quantification of Non-enzymatic Glycation of Vitreous Collagen using Non-invasive Light Scattering

¹Dunker S., ²Looke G., ³Dierks K., ²Wegener A.,
¹ (Troisdorf)
²Rheinische Friedrich-Wilhelms-Universität, Institut für Experimentelle Ophthalmologie (Bonn)
³Dierks und Partner System Technology (Hamburg)

Purpose: Type II collagen was isolated from bovine vitreous in an experimental model. Collagen was exposed to different concentrations of glucose over various periods of time. Afterwards, with the use of dynamic light scattering, non enzymatic glycosilation was measured in the specimen.
Method: Collagen type II was isolated from bovine vitreous. Isolated collagen was exposed to these concentrations of glucose: 10 mmol/l, 50 mmol/l, 133 mmol/l and 2800 mmol/l over 2, 4, 8 and 12 weeks. The molecule size was afterwards measured using near elastic light scattering (dynamic light scattering: DLS) non-invasively. As a controle we performed polyacrylamid gel electrophoresis and measurements of non-elastic light scattering with Raman spectroscopy.
Results: Cross binding of collagen increases with increase of glucose concentration and time. Raman spectroscopy and DLS showed comparable results.
Conclusions: With our experimental model we were able to simulate non enzymatic glycation of vitreous collagen in vitro. Glycation can be measured with DLS and Raman spectroscopy non invasively. Conclusions about human vitreous measurements with non invasive light scattering methods in situ, for example in diabetic vitreopathy, are drawn.

Copyright © Deutsche Ophthalmologische Gesellschaft, 2002. All rights reserved.

Last updated: 

Webdesign: SPALLEK.COM