Aktuelle Tagungsinformationen
News and Updates
Anmeldung zur Tagung
Registration
Hotelbuchung
Hotel Registration
Grußwort
Welcome address
Beteiligte Gesellschaften
Societies involved
Eröffnung des Kongresses
Opening Ceremony
Preise
Awards
Wissenschaftliches Programm
Scientific program
Posterpräsentationen
Poster Presentation
Kurse
Courses
Begleitende Veranstaltungen
Collateral Events
Rahmenprogramm
Social program
Jubiläumsparty
Jubilee Party
DOG Information
DOG Information
Allgemeine Informationen
General Information
Autorenindex
Index of Authors
Ausstellerliste
Exhibitors
Sponsoren
Sponsors
Teilnahmegebühren
Registration fees
Impressum
DOG Homepage
Optical Tomography of the Eye by Multiphoton Auto-fluorescence and SHG Imaging
König K., Krauß O., Riemann I.,
Friedrich-Schiller-Universität-Jena, Institut für Anatomie II, Zentrum für Lasermikroskopie (Jena)
Purpose: We evaluated the use of 80 MHz femtosecond laser pulses for high resolution imaging of the eye, in particular the cornea.
Method: We worked with porcine, mice and rat cadaver eyes on a modified inverse ZEISS LSM 410 with a tuneable Ti:Sa Laser MaiTai from SpectraPhysics as an external laser source and a special JENLAB interface consisting of a beam expander, motorized beam attenuator, fast shutter, power control and synchronization unit. The femtosecond laser beam was scanned in x,y and z direction. Simultaneously, the luminescence signal was recorded with a fast photomultiplier.
Results: Optical sectioning based on two-photon excited autofluorescence in the visible spectral range and second harmonic generation in the UV and the blue spectral range could be performed with submicron resolution. Interestingly, collagen revealed a strong SHG signal when excited with 750 nm - 850 nm femtosecond laser pulses at GW/cm2 light intensities. Tissue layers, single cells and intracellular fluorescent compartments could be clearly visulaized.
Conclusions: A novel high resolution imaging tool was developed based on multiphoton excited autofluorescence and second harmonic generation using near infrared femtosecond laser pulses.