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Intrastromal Surgery of the Cornea with 80 MHz Nanojoule Femtosecond Laser Pulses in the Near Infrared
Krauß O., Riemann I., König K.,
Friedrich-Schiller-Universität-Jena, Institut für Anatomie II, Zentrum für Lasermikroskopie (Jena)
Purpose: Recent investigations have demonstrated that fs-pulsed laser systems are applicable tools for refractive surgery. So far, amplified laser systems with µJ pulse energy have been used with the disadvantage of collateral damage by photodisruptive effects. We evaluated the use of sub-10 nJ laser pulses at 80 MHz repetition frequency for intrastromal surgery.
Method: We worked especially with porcine cadaver eyes on a modified inverse ZEISS LSM 410 with a tuneable Ti:Sa Laser MaiTai from SpectraPhysics as an external laser source and a special JENLAB interface consisting of a beam expander, motorized beam attenuator, fast shutter, power control and synchronization unit.
Results: In a depth up to 140 µm femtosecond laser pulses at 1-2 nJ and TW/cm² intensity produced highly precise submicron cuts. In addition, material within a ROI was ablated with high accuracy. Immediately after laser treatment the effects were visualized by 3D luminescence imaging with the same system using GW/cm² intensities. Following laser exposure eyes underwent examination by light, electron and force microscopy.
Conclusions: Compact turn-key NIR femtosecond-pulsed laser systems in combination with high N.A. optics can be used as a versatile tool for highly precise intrastromal surgery without further collateral damage.