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Adherence and Viability of Porcine-Lens Epithelial Cells on Poly(methyl methacrylate), Silicone and Soft Acrylate Intraocular Lenses
1Hesse Y., 1Lang G. K., 1Kampmeier J., 2Baldysiak-Figiel A., 1Lang G. E.,
1Universität Ulm, Augenklinik und Poliklinik (Ulm)
2Universität Ulm, Augenklinik und Poliklinik, Augenlabor (Ulm)
Purpose: To evaluate lens epithelial cell (LEC) adhesion and viability on three different intraocular lens (IOL) materials.
Methods: The IOL materials tested were poly(methylmethacrylate) (PMMA) (Pharmacia), silicone (Pharmacia) and hydrophobic acrylate (AcrySof, Alcon Surgical). Primary porcine LECs (5x104 cells/ml) were cultured on four discs of each material over a period of 10 days. Cells grown on untreated wells of a 96-well microtiter plate served as a control. Cell adhesion was documentated on days 1,2,3,4,6,8,10. A cell viability test using the LIVE/DEAD kit (Molecular Probes) was carried out at the end of the investigation. Experiments were run in triplicate. Statistical analysis was performed using Student´s unpaired t-test.
Results: Confluent cell growth was reached on the soft acrylate discs and the control wells within one day and remained stable during the experiment. Only a few round cells were adherent on PMMA and silicone discs and the number reduced during the experiment. The viability test revealed 105.83 ± 21.78% (mean ± SD) alive cells in control wells and 81.79 ± 51.74% on acrylate discs, which was not significant (p=0.1589). Significantly lower relative numbers of alive cells (p>0.0001) were found on PMMA (-0.62 ± 2.24%) and silicone (21.47 ± 8.42%) discs. The percentage of dead cells was significanty higher (p<0.0001) on acrylate discs (29.69 ± 12.61%) compared to control (-1.49 ± 4.13%). The ratios of dead cells on PMMA discs and silicone discs were -14.51 ± 7.23% (p<0.0001) and 15.83 ±16.28% (p=0.0036).
Conclusions: Significantly fewer cells grew on PMMA and silicone discs compared to control. Confluent cell growth and numbers of viable cells comparable to control were observed on AcrySof discs after a culture period of 10 days with a significant higher percentage of dead cells.
Supported by Alcon and Pharmacia.