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Documentation and Analysis of Infiltrating Cells in the Anterior Chamber and Vitreous via Digital Video Recording
1Becker M., 1Mackensen F., 2Auffarth G. U., 2Völcker H. E.,
1Ruprecht-Karls-Universität Heidelberg, Universitäts-Augenklinik, Interdisziplinäres Uveitis-Zentrum (Heidelberg)
2Ruprecht-Karls-Universität Heidelberg, Universitäts-Augenklinik (Heidelberg)
Purpose: Infiltrating cells in the anterior chamber or vitreous are a prerequisite for the diagnosis of intraocular inflammation. Clinically the amount of cells is guessed semiquantitatively. Laser flare meter is an objective measure for inflammation activity, however the number of cells seems to be much better correlated with activity of inflammation than the protein exudate. Therefore, a method is needed to study the number of free cells in the anterior chamber or vitreous objectively.
Methods: The anterior chamber and vitreous of five patients with anterior uveitis or vitritis were recorded with a standard setup: 1x1 mm light beam, maximum light intensity, 60 degree slit angle, and 25-fold magnification. Videos were recorded with a 3-chip-camera (Sony) and a digital recording system.
Results: With this technology cells in the anterior chamber as well as the vitreous could be detected, recorded and quantified.
Discussion: Single cells in the anterior chamber and vitreous of uveitis patients have been recorded with this non-invasive technique. Future projects include a calibration of the methodology and automatic cell counting. This method could be used to study drugs objectively, e.g. the effect of eye drops in iritis patients as well perioperatively in cataract patients.