Programm & Abstracts                 "Innovationen in der Augenheilkunde"

Aktuelle Tagungsinformationen
   News and Updates

Anmeldung zur Tagung
   Hotel Registration
   Welcome address
Beteiligte Gesellschaften
   Societies involved
Eröffnung des Kongresses
   Opening Ceremony
Wissenschaftliches Programm
   Scientific program
   Poster Presentation
Begleitende Veranstaltungen
   Collateral Events
   Social program
   Jubilee Party
DOG Information
   DOG Information
Allgemeine Informationen
   General Information
   Index of Authors
   Registration fees

DOG Homepage

Expression of the TGF-b Activating Molecule Thrombospondin-1 in the Trabecular Meshwork

1Ohlmann A., 1Flügel-Koch C., 2Tamm E.,
1Friedrich-Alexander-Universität, Institut für Anatomie, Molekulare Anatomie & Embryologie (Erlangen)
2Friedrich-Alexander-Universität, Anatomisches Institut, Molekulare Anatomie und Embryologie (Erlangen)

Purpose: In glaucomatous eyes, aqueous humor contains increased levels of transforming growth factor-β2 (TGF-β2). Most, if not all TGF-b2 in the aqueous humor is present in a latent, inactive form. Only little is known where and if TGF-β2 is activated in the anterior eye. Since thrombospondin-1 (TSP-1) is known to be a potent endogenous activator of TGF-β2, we investigated the expression of TSP-1 in the human and mouse eye.
Method: Normal and glaucomatous human eyes as well as normal and TSP-1 deficient mice eyes were examined by immunohistochemistry and RT-PCR. In addition northern and western blot analyses of cultured human trabecular meshwork cells were performed.
Results: In human and mouse eyes, the trabecular meshwork (TM) was intensely labelled for TSP-1. A weak signal for TSP-1 was observed in Bruch´s and Descemet´s membranes. TSP-1 deficient mice that were used as negative controls, showed no immunostaining for TSP-1. No differences in TSP-1 localization was observed between normal human eyes and those with primary open angle glaucoma (POAG). In cultured human trabecular meshwork cells, an expression of TSP-1 was seen in western and northern blot analyses. After incubation of the cells with activated TGF-β1, an increase in mRNA for TSP-1 was observed.
Conclusions: TSP-1 in the TM might act as a potent activator of TGF-β2 in the aqueous humor and mediate any local effects of TGF-β2 on the outflow tissues in normal eyes and those with POAG.