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Comparison of Scotopic Pupil Measurements using Colvard Pupilometer and Sekundo´s Slitlamp Green Light Test
Nietgen G. W., Sekundo W.,
Philipps-Universität Marburg, Medizinisches Zentrum für Augenheilkunde (Marburg)
Purpose: to compare the accuracy and the reproducibility of scotopic pupil measurements using to different methods
Methods: We developed a simple test to measure a scotopic pupil diameter using a narrow slit of light through a green filter of the Haag-Streit slitlamp. 100 eyes of 50 refractive surgery candidates were prospectively examined by two independent investigators using both the Colvard pupilometer (Oasis/USA) and Sekundo´s slitlamp green light test. Results were compared using All Pairwise Multiple Comparison Procedures (Student-Newman-Keuls) Method [SigmaStat/Jandel Scientific]. Patients´mean age was 36.3 years with a male:female ratio of 16:34. The colour of the iris was considered blue 36 individuals, being brown in the remainder.
Results: Reproducibility: The right eye mean pupil diameter using Sekundo´s method was 6.4mm (± 0.9) measured by both the first [Slit 1R] and the second [Slit 2R] investigator. The left eye mean pupil diameter was 6.5mm (± 1) [Slit 1L] and 6.35 mm (± 1) [Slit 2L] respectively. Following measurements were obtained with the Colvard pupillometer: [Colv1R] = 6.25 ± 0.85mm; [Colv2R] = 5.99 ± 1mm; [Colv1L] = 6.15 ± 0.91; [Colv2L] = 6.05 ± 0.93. There were no statistically significant differences between the two investigators within the same method of examination. Comparability: The data of both investigators were combined to form 4 groups: [Slit R] = 6.4 ± 0.96 mm; [Slit L] = 6.4 ± 1 mm; [Colv R] = 6.05 ± 0.9mm; [Colv L] = 6.1 ± 0.9mm. There was a significant difference (p=0.024) between the two methods for both right and left eyes.
Conclusions: Sekundo´s slitlamp green light test provides similarly reproducible results for the measurement of the scotopic pupil diameter as the commercially available Colvard pupilometer. However, particularly for the large pupils, the slitlamp green light measurements are significantly higher (up to 0.35mm) then those with Colvard´s device. The difference might be related to the technique itself and/or to the fact that Colvard´s method measures the horizontal diameter, whereas Sekundo´s method provides the vertical pupil hight.