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Characterization of Morphology and Intercellular Communication Systems of Human Keratocytes in an Artificial Bio-matrix
Denk P. O.,
Eberhard-Karls-Universität Tübingen, Universitäts-Augenklinik, Abt. I (Tübingen)
Purpose: To investigate the morphology and intercellular communication systems of human keratocytes in a collagen-glycosaminoglycan matrix.
Method: Human keratocytes were grown in explant culture from corneas which did not fulfill quality criteria for transplantation. After the second passage, keratocytes were seeded into a collagen-glycosaminoglycane matrix, which was cross-linked using 1-ethyl-3(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS). Morphology and cell-cell contacts of keratocytes were examined using immunohistochemical techniques.
Results: Keratocytes in the collagen-glycosaminoglycane matrix were spindle-shaped and formed multiple pseudopodiae.The high expression density of connexin 43 indicates the formation of gap junctions between keratocytes.
Conclusions: The morphology and intercellular communication systems of human keratocytes in an artificial biomatrix resemble the original phenotype of keratocytes in the human cornea. An application of this biomatrix as an artificial cornea seems feasible in the future.