Programm & Abstracts                 "Innovationen in der Augenheilkunde"

Aktuelle Tagungsinformationen
   News and Updates

Anmeldung zur Tagung
   Registration
Hotelbuchung
   Hotel Registration
Grußwort
   Welcome address
Beteiligte Gesellschaften
   Societies involved
Eröffnung des Kongresses
   Opening Ceremony
Preise
   Awards
Wissenschaftliches Programm
   Scientific program
Posterpräsentationen
   Poster Presentation
Kurse
   Courses
Begleitende Veranstaltungen
   Collateral Events
Rahmenprogramm
   Social program
Jubiläumsparty
   Jubilee Party
DOG Information
   DOG Information
Allgemeine Informationen
   General Information
Autorenindex
   Index of Authors
Ausstellerliste
   Exhibitors
Sponsoren
   Sponsors
Teilnahmegebühren
   Registration fees
Impressum



DOG Homepage

Effects of Allopurinol on T-cells and Cytokine Profile in Experimental Autoimmune Uveitis (EAU)

1Repp A., 1Grus F. H., 1Hambek D., 2Augustin A. J., 1Pfeiffer N.,
1Johannes-Gutenberg-Universität Mainz, Universitäts-Augenklinik (Mainz)
2Städtisches Klinikum Karlsruhe, Augenklinik (Karlsruhe)

Purpose: Allopurinol is a well-known antioxidative and anti-inflammatory agent. Previous studies using different animal models of uveitis provide evidence that allopurinol changes the autoantibody repertoire and influences the T-cell proliferation. The purpose of this study was to assess the effect of allopurinol on the cytokine profile of T-cells in animals suffering from EAU.
Method: 11 male Lewis rats were divided into three groups. 6 animals were immunized with crude retinal extract, Freund`s Adjuvans and pertussis toxine: 3 of them were left untreated (EAU) and 3 received allopurinol treatment (50 mg/kg bw i.v.; ALLO). 5 healthy rats served as control (CONTROL). Furthermore a subset of T-cells of each group was treated in vitro with allopurinol. Intracellular staining was performed using anti-cytokine fluorochrome-conjugated antibodies (anti- interleukin 2,4,6 and interferon gamma). Flow cytometric analysis (FACScan) allowed the characterization of individual cytokine-producing T-lymphocytes.
Results: The FACScan confirmed a significant (p<0,05) reduction of cytokine production (interleukin 2,4 and 6) after systemically applied allopurinol as well as after in vitro treatment for 72 h in all groups. The analysis of INF-gamma revealed different results: application of allopurinol in vitro for 24 h resulted in a reduction of INF-gamma positive cells in the groups EAU and ALLO. However, the production of INF-gamma increased after 72 h treatment in these groups.
Conclusions: In this study allopurinol could demonstrate immunological effects in the cytokine production of T-cells. Regarding the effect of allopurinol on INF-gamma production allopurinol not only leads to a general immunsuppression, rather an immunmodulation. Along with its antioxidative effects described in previous studies, allopurinol might be of useful therapeutic value in the treatment of uveitis.
DFG (AU 126/2)

Zurück/Back