|Programm||"Degeneration und Regeneration– Grundlagen, Diagnostik und Therapie"|
AGE´s in Age Related Macular Degeneration
Hoffmann S., Yasukawa T., Friedrichs U., Eichler W., Wiedemann P.
Age related macular degeneration is a common cause of blindness in the elderly. The pathologic mechanisms of the disease are not totally evaluated. Angiogenic cytokines are of special importance for the disease but also advanced glycation endproducts (AGEs) seem to play a role in the disease process. We evaluated the effects of AGEs on the proliferation and migration of choroidal endothelial cells (CECs) and retinal pigmented epithelial cells (RPEs). In addition, the AGE effect on the expression of the matrix-metalloproteinase-2 by CECs and the expression of vascular endothelial growth factor (VEGF) by RPE cells was investigated. AGEs enhance the proliferation and migration of CECs and RPE cells in vitro. Furthermore, AGEs enhance the expression of vascular endothelial growth factor (VEGF) by RPE cells and induce the expression of the matrix metalloproteinase-2 by CECs. The in vivo effects of AGEs were investigated in a rabbit model by use of AGE impregnated microspheres. AGEs induce in vivo the formation of drusen like structures in Bruch`s membran and choroidal neovascularization. Therefore, AGEs may be of importance for the induction of AMD.