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Abstract
Abstract
Trophic Capacity of Platelet Lysate on Corneal Epithelial Cells An Alternative to Autologous Serum Eye Drops for Ocular Surface Disease?
Geerling G., Harloff S., Liu L., Wedel T., Hartwig D.
Dept. of Ophthalmology, Medical University, Luebeck
Purpose: Eye drops prepared from serum have been successfully used to treat ocular surface disorders such as dry eyes or persistent epithelial defects. Their beneficial effect was attributed to a variety of growth factors present in serum. Platelets are a major source of growth factors in serum. They are available at adjustable concentrations in platelet rich suspensions from blood banks and following stimulation with thrombin release their growth factor content. We examined the epitheliotrophic capactiy of platelet lysates in comparison with serum in an in-vitro model of human corneal epithelial cells.
Method: Platelet concentrates with 4 x 109 thrombocytes / ml and full blood samples were obtained of 10 healthy donors each and prepared under standardised clotting and centrifugation conditions. In these EGF, HGF, FGF PDGF, TGF-b, fibronectin, as well as vitamin A and E were quantified by means of routine ELISA- or HPLC-technology. SV-40 immortalised human corneal keratinocytes were cultured in 96-well plates at 37°C, 5% CO2 with a fully defined culture medium. At 30% confluency the culture medium was substituted by one of the test preparations. Proliferation of cell cultures was quantified by means of a luminescence-based ATP-Assay in dose-response experiments. A colony dis