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Abstract
Abstract
Carbonylgroups Introduced in Proteins by Oxidative Modifications in Normal Macula and CNV-Lesions by Exudative AMD
Hermans P.1,3, Lommatzsch A.2, Wasmuth S.3, Bauer D.1 , Müller D.4, Bornfeld N.3, Pauleikhoff D.2
1Ophtha-Lab, 2Dept. Ophthalmology, St. Franziskus-Hospital, Muenster; 3University Eye Hospital, 4Dept. Microbiology, University Essen
Purpose: Oxidative stress can modify proteins that frequently result in functional changes, such as impaired enzymatic activity, decreased/increased DNA binding activities of transcription factors, and decreased susceptibility to proteolytic degradation. The aim of the present study was to detect carbonyl groups introduced in protein side chains by reactive oxygen species (ROS) or by reaction with aldehydes (malondialdehyd) in normal macula and choroidal neovascularisation membranes (CNV) from patients with exudative age-related macular degeneration (AMD).
Method: Macula specimen of 25 donor eyes and 20 surgically excised exudative AMD lesions, with choroidal neovascularisation were examined. Carbonyl residues were detected by reaction with 2,4-dinitrophenylhydrazine (DNPH) generating dinitrophenylhydrazones. The detection was processed with an antidinitrophenylhydrazone antibody followed by the immunohistochemical ABC-method.
Results: Protein carbonyls were detected in endothelial cells of the choriocapillaris and only rare in the retinal pigment epithelium (RPE) in normal macula. Bruch