Hotelbuchung
Hotel Registration
Grußwort
Welcome address
Beteiligte Gesellschaften
Societies involved
DOG Information
DOG Information
Eröffnung des Kongresses
Opening Ceremony
Preise
Awards
Ablauf der Tagung 2003
General overview of congress
Lageplan der Räumlichkeiten
Map of Congress Center
Wissenschaftliche Themen
Scientific topics
Symposien
Symposia
Wissenschaftliches Programm
Scientific program
Posterpräsentationen
Poster Presentation
Kurse
Courses
Begleitende Veranstaltungen
Accompanying program
Arbeitssitzungen
Working sessions
Rahmenprogramm
Social program
Allgemeine Informationen
General Information
Autorenindex
Index of Authors
Industrieaussteller
Commercial exhibitors
Sponsoren
Sponsors
Impressum
DOG Homepage
Abstract
Abstract
Vitreous Serum Albumin as a Cause of Precipitate Formation and Emulsification under Vitreous Tamponade with Hydrofluorocarbons
Hoerauf H.1, Moll R.2, John H.3, Laqua H.1
1Department of Ophthalmology, University of Luebeck; 2IPF Pharmaceuticals GmbH, Hannover; 3Institue for Biochemistry, University of Luebeck
Purpose: To investigate the role of precipitate formation and emulsification after ocular endotamponade with hydrofluorocarbons (HFCL).
Method: A) Intraoperative specimen of precipitates under HFCL-tamponade were analyzed by electrophoresis and MALDI-TOF analysis. Protease-digested peptide fragments have been identified by N-terminal Edman-sequence-analysis. B) Human serumalbumin (HSA) and different vitreous endotamponades (O62, O62-Oligomer, F6H8, PFD, silicone oil) were rotated in transparent vials.
Results: A) Chemical analysis of the "in vivo" precipitate containing sample detected modified HSA. B) Precipitate formation was only observed mixing HSA with O62-Oligomer. Dispersion/emulsification alone was observed in O62-Oligomer > O62 > F6H8 > Silicone oil. The size and transparency of droplets differed markedly.
Conclusions: The increased amount of HSA in PVR-cases with severe blood aqueous barrier breakdown can precipitate in presence of certain fluorocarbons and cause emulsifications of HFCL in variable extent. HSA may be modified by fluorocarbons and act as an antigen causing severe inflammatory