Expression of Angiogenic Factors by Photodynamic Therapy
Rudolf M.1, Michels S.2, Schlötzer-Schrehardt U.3, Schmidt-Erfurth U.2
University Hospital Schleswig-Holstein, 1Campus Kiel, 2Campus Luebeck; 3Department of Ophthalmology, University of Erlangen-Nuremberg, Erlangen
Purpose: To evaluate the influence of photodynamic therapy (PDT) on the regulation of angiogenic factors (vascular endothelial growth factor (VEGF), VEGF receptor 3 (VEGFR-3) and pigment epithelium derived factor (PEDF).
Method: Verteporfin-PDT using verteporfin with the recommended standard parameters was applied to intact areas of the perimacular region. Human eyes of patients scheduled for enucleation due to untreatable malignancy served as study eyes (n=4), age-matched donor eyes were used as controls (n=4). Treatment areas were classified by ophthalmoscopy, fluorescein (FA) and indocyanine green angiography (ICGA). LM and EM histology was performed after enucleation at 1 week. Immunhistochemistry using specific antibodies against VEGF, VEGFR-3 and PEDF was performed in PDT-treated areas and untreated collateral areas in study eyes as well as untreated areas of control eyes. Specimen were fixed in 4% paraformaldehyde, 1% glutaraldehyde and embedded in paraffine. Four µm thick sections were stained using the peroxidase labeled streptavidin-biotin method.
Results: All PDT-treated areas demonstrated a characteristic choroidal hypofluorescence by FA/ICGA. LM/EM histology revealed a selective alteration of chori
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