|Programm||"Degeneration und Regeneration– Grundlagen, Diagnostik und Therapie"|
Efficient Non-viral Transfection of Neural Stem Cells
Richard I.1, Ader M.1, Sytnyk V.2, Richard G.1, Schachner M.2, Bartsch U.2
Purpose: Cell therapy (i.e. replacement of degenerated or dysfunctional cells) with neural stem cells (NSCs) is discussed as a possible strategy to treat neurodegenerative disorders. In addition, transplantations of genetically engineered NSCs might be of use to target therapeutic gene products to the diseased nervous system. Here, we describe a novel non-viral transfection technique which allows the efficient transfection of in vitro expanded NSCs derived from the spinal cord of mouse embryos.