Hotelbuchung
Hotel Registration
Grußwort
Welcome address
Beteiligte Gesellschaften
Societies involved
DOG Information
DOG Information
Eröffnung des Kongresses
Opening Ceremony
Preise
Awards
Ablauf der Tagung 2003
General overview of congress
Lageplan der Räumlichkeiten
Map of Congress Center
Wissenschaftliche Themen
Scientific topics
Symposien
Symposia
Wissenschaftliches Programm
Scientific program
Posterpräsentationen
Poster Presentation
Kurse
Courses
Begleitende Veranstaltungen
Accompanying program
Arbeitssitzungen
Working sessions
Rahmenprogramm
Social program
Allgemeine Informationen
General Information
Autorenindex
Index of Authors
Industrieaussteller
Commercial exhibitors
Sponsoren
Sponsors
Impressum
DOG Homepage
Abstract
Abstract
Efficient Non-viral Transfection of Neural Stem Cells
Richard I.1, Ader M.1, Sytnyk V.2, Richard G.1, Schachner M.2, Bartsch U.2
1University Eye Clinic Hamburg Eppendorf and 2Zentrum für Molekulare Neurobiologie, Hamburg
Purpose: Cell therapy (i.e. replacement of degenerated or dysfunctional cells) with neural stem cells (NSCs) is discussed as a possible strategy to treat neurodegenerative disorders. In addition, transplantations of genetically engineered NSCs might be of use to target therapeutic gene products to the diseased nervous system. Here, we describe a novel non-viral transfection technique which allows the efficient transfection of in vitro expanded NSCs derived from the spinal cord of mouse embryos.
Method: NSCs were isolated from the spinal cord of wild-type, L1-deficient or enhanced green fluorescent protein- (EGFP) transgenic mouse embryos, and expanded in vitro in the presence of epidermal growth factor and fibroblast growth factor-2. Cells from the third to sixth passage were transfected with the reporter gene EGFP or the cell recognition molecule L1 using the NucleofectorTM technique (Amaxa Biosystems, Köln, Germany). Transfected cells were analysed between one day and 3.5 weeks after transfection in vitro, and up to 3.5 weeks after intraretinal transplantation in vivo.
Results: Quantitative analysis of wild-type cultures and L1-deficient cultures one day after transfection with EGFP and L1, respective