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Abstract
Abstract
Do Cell Culture Media Supplements Influence the Survival of Retinal Ganglion Cells?
Knop C. O.1, Li Y.2, Vorwerk C. K.1, Nickells R. W.2
1Augenklinik, Otto-von-Guericke University, Magdeburg; 2Ophthalmology and Visual Sciences, University of Wisconsin, Madison/USA
Purpose: Survival and regeneration of retinal ganglion cells after different damaging stimuli depend on a variety of growth factors and supplements. Several studies showed that a cocktail of growth factors and agents is needed for optimal conditions. Various ocular cell types produce theses factors, often in response to some traumatic damage. In this study we used a mixture of different molecules and factors which are known to enhance growth and regeneration in vitro to show the same effect in vivo. Some commercially available supplements were tested in a CB6F1 mouse model of optic nerve crush.
Method: Supplements, ranging from fetal calf serum to a specialized additive for neurons (N2-supplement) were obtained from commercial sources. Eyes received an intravitreal injection of 2 µL of undiluted or diluted (to 10% in BSS) supplement 24 hours before optic nerve crush. Two weeks after crush, the mice were sacrificed and the numbers of surviving cells were counted from Nissl-stained wholemounts of both control and experimental eyes.
Results: Untreated eyes showed a 30 ± 6.2% (mean ± sem) loss of cells 2 weeks after optic nerve crush. The majority of supplements tested did not significantly attenuate cell loss (Student t-test