Hotelbuchung
Hotel Registration
Grußwort
Welcome address
Beteiligte Gesellschaften
Societies involved
DOG Information
DOG Information
Eröffnung des Kongresses
Opening Ceremony
Preise
Awards
Ablauf der Tagung 2003
General overview of congress
Lageplan der Räumlichkeiten
Map of Congress Center
Wissenschaftliche Themen
Scientific topics
Symposien
Symposia
Wissenschaftliches Programm
Scientific program
Posterpräsentationen
Poster Presentation
Kurse
Courses
Begleitende Veranstaltungen
Accompanying program
Arbeitssitzungen
Working sessions
Rahmenprogramm
Social program
Allgemeine Informationen
General Information
Autorenindex
Index of Authors
Industrieaussteller
Commercial exhibitors
Sponsoren
Sponsors
Impressum
DOG Homepage
Abstract
Abstract
TGFb Effects on Primary Human Tenon Fibroblasts in Regard to Ocular Wound Healing
Meyer-ter-Vehn T.1, Grehn F.1, Knaus P.2
1Universitäts-Augenklinik Würzburg; 2Institut für physioloigsche Chemie II, Universität Würzburg
Purpose: A major complication after trabeculectomy consists in the postoperative scarring with consecutive therapeutic failure. The growth factor transforming growth factor b (TGFb) plays a pivotal role in the process of wound healing in the eye, TGFb2 appears to be the predominant isoform. This study focuses on the effect of TGFb isoforms on primary human tenon fibroblasts (HTF) as well as on the fibroblast cell line NIH 3T3 with special regard to ocular wound healing.
Method: Primary human tenon fibroblasts (HTF) were expanded in culture - their TGFb receptor status was assessed by RT-PCR and by immunohistochemistry. HTFs and NIH 3T3 cells were stimulated with different concentrations of TGFb isoforms and activin A for various time periods. Activation of the SMAD signalling pathway was determined by western blot using phosphospecific antibodies. Expression of SMAD7, a negative regulator of the SMAD signal transduction, was assessed by western blot. In view of the process of ocular wound healing, HTFs were stimulated for 2-3 days and synthesis of alpha smooth muscle actin as well as fibronectin marker genes in the process o