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Abstract
Abstract
Characterisation of L-type Ca2+ channels in Human Trabecular Meshwork Cells and the effect of Endothelin-1
Steinhausen K.1, Strauß O.1, Wiederholt M.2
1Experimentelle Ophthalmologie, Universitätsklinikum Hamburg Eppendorf; 2Institut für Klinische Physiologie, Universitätsklinikum Benjamin Franklin, Berlin
Purpose: The trabecular meshwork (TM) influences aqueous humor outflow resistance and thus the level of IOP. L-type Ca2+ channels have been shown to influence [Ca2+]i and the contractility of the TM. Application of Endothelin-1 (ET-1) activates TM contractility and leads to an elevation of [Ca2+]i. Thus, we examined possible effects of endothelin on L-type Ca2+ currents of human trabecular meshwork cells (HTM) to understand the signal transduction pathway of ET-1.
Method: L-type Ca2+ currents and the effects of ET-1 on L-type Ca2+ currents of cultured HTM cells were investigated using the patch-clamp technique.
Results: In the presence of 10 mM Ba2+ as charge carrier, depolarization of HTM cells from a holding potential of -70 mV in 10 mV voltage steps of 150 ms duration to +20 mV led to an activation of inward currents at potentials more positive than about -30 mV. These currents were identified as L-type Ca2+ currents by means of their voltage dependent inactivation kinetics and dihydropyridine sensitivity. The L-type channel opener BayK8644 lead to an increase to 150 % (n= 7) of control current and