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Abstract
Abstract
Cloning of a New Splice Variant of the Cav1.3 (a1D) L-type Ca2+ Channel Expressed by Retinal Pigment Epithelial Cells
Wimmers S., Strauss O.
Experimentelle Ophthalmologie Universitätsklinikum Hamburg-Eppendorf
Purpose: The L-type Ca2+ channels in retinal pigment epithelial (RPE) cells are involved in the etiology of retinal and macula degeneration. These Ca2+ channels are the target of different protein kinases and are further modified by the interaction with additional subunits. Thus, it is of great interest to know the exact molecular structure of the channels in RPE cells.
Method: We extracted mRNA from the ARPE-19 cell line. This mRNA was used as a template for the amplification and cloning of the Cav1.3 Ca2+ channel.
Results: The sequence analysis of the amplified gene revealed a new splice-variant of the Cav1.3 Ca2+ channel lacking an exon in the C-terminus. As this lacking exon is coding for an amino acid sequence possibly phosphorylated by different protein kinases, this splicing might be responsible for the unique reponsiveness of L-type Ca2+ currents to protein kinases in RPE cells.
Conclusions: Since much work is already done to identify splice-variants of L-type Ca2+ channels in other tissues the described new splice-variant is probably a RPE specific splice-variant suitable to the task in these cells.