Programm                 "Degeneration und Regeneration– Grundlagen, Diagnostik und Therapie"


Hotelbuchung
   Hotel Registration
Grußwort
   Welcome address
Beteiligte Gesellschaften
   Societies involved
DOG Information
   DOG Information
Eröffnung des Kongresses
   Opening Ceremony
Preise
   Awards
Ablauf der Tagung 2003
   General overview of congress
Lageplan der Räumlichkeiten
   Map of Congress Center
Wissenschaftliche Themen
   Scientific topics
Symposien
   Symposia
Wissenschaftliches Programm
   Scientific program
Posterpräsentationen
   Poster Presentation
Kurse
   Courses
Begleitende Veranstaltungen
   Accompanying program
Arbeitssitzungen
   Working sessions
Rahmenprogramm
   Social program
Allgemeine Informationen
   General Information
Autorenindex
   Index of Authors
Industrieaussteller
   Commercial exhibitors
Sponsoren
   Sponsors
Impressum



DOG Homepage


Abstract
Abstract

Transplantation of Human Corneal Endothelial Cells as Potential Therapy of Corneal Endothelial Dystrophies

Aboalchamat B.1, Böhnke M.2, Engelmann K.3, Bednarz J.1
1Ophthalmology Department, Univerisity of Hamburg; 2Ophthalmic and Laser Center, Hamburg; 3Ophthalmology Department, University of Dresden

Purpose: Our previous studies showed that transplantation of human corneal endothelial cells onto organ cultured corneas led to the establishment of a morphological and functional intact endothelium. In this study we developed a new technique to transplant human corneal endothelial cells onto human corneas in whole globes.
Method: We used porcine eyes as well as human eyes that were not suitable for donor cornea preparation. Following paracentesis, the native corneal endothelium was removed by scratching with polishing cannulas and simultaneous rinsing with isotonic saline solution. Subsequently air was inflated into the anterior chamber. Suspensions of 250 000 cells of a SV40 transfected cell line in 150 ml culture medium were injected into the air inflated anterior chamber of donor globes with the cornea downside. The globes were kept in this position for different incubation periods. Subsequently the corneas were prepared for organ culture. The transplanted cells were identified by immunochemical proof of SV40 T-antigen expression and scanning electronmicroscopy.
Results: The use of polishing cannulas led to a complete removal of the native corneal endothelium


Zurück | Back