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Abstract
Abstract
Hepatitis C Testing in Cornea Donors by Polymerase Chain Reaction
Priglinger S.1, Neubauer A. S.1, Eberle J.2, Alge C.1, Kampik A.1, Welge-Lüßen U.1
1Department of Ophthalmology, 2Max-von-Pettenkofer Institut, Ludwig-Maximilians-University, Munich
Purpose: Screening all cornea donors for hepatitis C (HCV) by polymerase chain reaction (PCR) testing has recently become mandatory in Germany. Blood sample analysis of donors with long post mortem times is discussed controversially. Therefore the aim of this study was to evaluate whether post-mortem donor screening by HCV-PCR is a feasible and suitable method to detect HCV positive donors.
Method: In 100 potential donors hepatitis C testing by conventional enzyme-linked immunosorbent assay (ELISA) and PCR was performed. Post-mortem time was in mean 22.0±15.4 hours (range 0-72 hours). Donor liver changes were histological activity index (HAI). PCR and ELISA results were correlated to autopsy results of the liver.
Results: ELISA testing for Hepatitis C was positive in 3 cases. In two of those cases no Hepatitis C RNA could be detected by PCR. In all of those ELISA positive donors liver changes were observed on autopsy (HAI 3 respectively 4). In 4 other donors Hepatitis C PCR was positive, while ELISA testing was negative. 3 of these donors showed pathologic liver changes (HAI 4 respectively 5). One of the PCR positive donors showed no pathologic liver changes. All 4 donors were excluded based on the results of PCR testing.
Conc