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Abstract
Abstract
Hypoxia Increases the Expression of CTGF and PAI-1 in Retinal Pigment Epithelium
Welge-Lüßen U.1, R. Fuchshofer R.2, Kampik A.1, Lütjen-Drecoll E.2
1Department of Ophthalmology, Ludwig-Maximilians University, München
2Anatomy II, Friedrich-Alexander University, Erlangen-Nürnberg, Erlangen
Purpose: Early age related macular degeneration (ARMD) is characterised by atrophia of the choroid and the thickening of Bruch´s membrane by the accumulation of extracellular matrix (ECM). These findings could be related to hypoxia of the outer retina and retinal pigment epithelium (RPE). One factor known to increase the formation of ECM is connective tissue growth factor (CTGF). Additional CTGF has been proposed to be involved in the angiogenesis. The enzyme plasminogen activator inhibitor-1 (PAI-1) inhibits the degradation of ECM. In the present study we have investigated the effect of hypoxia on the expression of CTGF and PAI-1 in cultivated human RPE cells.
Method: The expression of CTGF and PAI-1 in native RPE cells of 6 donors was investigated by RT-PCR, northern-blot and western-blot analysis. Cultivated RPE cells from 5 human donors of the third passage were: 1. 4 - 12 hours under hypoxic conditions (1% O2) incubated 2. 1 - 16 hours re-oxygenated. The induction of CTGF and PAI-1 were quantified by using western-blot and northern-blot analysis.
Results: Native RPE cells constitutively express CTGF und PAI-1. Hypoxia increases the expression of CTGF in cultivated RPE cells a