Programm                 "Degeneration und Regeneration– Grundlagen, Diagnostik und Therapie"


Hotelbuchung
   Hotel Registration
Grußwort
   Welcome address
Beteiligte Gesellschaften
   Societies involved
DOG Information
   DOG Information
Eröffnung des Kongresses
   Opening Ceremony
Preise
   Awards
Ablauf der Tagung 2003
   General overview of congress
Lageplan der Räumlichkeiten
   Map of Congress Center
Wissenschaftliche Themen
   Scientific topics
Symposien
   Symposia
Wissenschaftliches Programm
   Scientific program
Posterpräsentationen
   Poster Presentation
Kurse
   Courses
Begleitende Veranstaltungen
   Accompanying program
Arbeitssitzungen
   Working sessions
Rahmenprogramm
   Social program
Allgemeine Informationen
   General Information
Autorenindex
   Index of Authors
Industrieaussteller
   Commercial exhibitors
Sponsoren
   Sponsors
Impressum



DOG Homepage


Abstract
Abstract

Hypoxia Increases the Expression of CTGF and PAI-1 in Retinal Pigment Epithelium

Welge-Lüßen U.1, R. Fuchshofer R.2, Kampik A.1, Lütjen-Drecoll E.2
1Department of Ophthalmology, Ludwig-Maximilians University, München
2Anatomy II, Friedrich-Alexander University, Erlangen-Nürnberg, Erlangen

Purpose: Early age related macular degeneration (ARMD) is characterised by atrophia of the choroid and the thickening of Bruch´s membrane by the accumulation of extracellular matrix (ECM). These findings could be related to hypoxia of the outer retina and retinal pigment epithelium (RPE). One factor known to increase the formation of ECM is connective tissue growth factor (CTGF). Additional CTGF has been proposed to be involved in the angiogenesis. The enzyme plasminogen activator inhibitor-1 (PAI-1) inhibits the degradation of ECM. In the present study we have investigated the effect of hypoxia on the expression of CTGF and PAI-1 in cultivated human RPE cells.
Method: The expression of CTGF and PAI-1 in native RPE cells of 6 donors was investigated by RT-PCR, northern-blot and western-blot analysis. Cultivated RPE cells from 5 human donors of the third passage were: 1. 4 - 12 hours under hypoxic conditions (1% O2) incubated 2. 1 - 16 hours re-oxygenated. The induction of CTGF and PAI-1 were quantified by using western-blot and northern-blot analysis.
Results: Native RPE cells constitutively express CTGF und PAI-1. Hypoxia increases the expression of CTGF in cultivated RPE cells a


Zurück | Back