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Abstract
Abstract
In vitro Cornea Model A New Opportunity in Organ Culture and a Test System Replacing in vivo Experiments
Nietgen A. M.1, Keller-Kuckelkorn G. K.1, Salla S.1, Kondring B.1, Frentz W.2, Reim M.1, Schrage N. F.1
1Augenklinik des Universitätsklinikums Aachen
2Institut für Aerodynamik der RWTH Aachen
Purpose: Corneal perfusion chambers are important tools in the development and assessment of ophthalmic drugs. On the other side we will present another opportunity for long time cornea perfusion. The aim of our study was to design and test a modified perfusion chamber suitable for topical drug application to the isolated rabbit cornea and additionally continuous monitoring of endothelial and epithelial cell function.
Method: We designed a PLEXIGLAS® perfusion chamber to clamp corneas to a horizontal convex device suitable for topical agent delivery and additionally offering suitable conditions for isolated in our experiments rabbit cornea. Endothelial function was assessed by pachymetry, epithelial function and surface morphology was assessed by fluorescein application and following slit-lamp examination. The low volume eye testing (LVET) method was used to dip the cornea in 10 ml of test substance for a defined time.
Results: Corneas maintained a functionally intact endothelial monolayer during perfusion periods up to 22 days. The epithelial function was well preserved.
Conclusions: The new c