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Abstract
Abstract
Antimicrobial Peptides in Human Lacrimal Gland, Cornea and Conjunctiva Beta-defensin 2 and Psoriasin are Induced in Dry Eye
Paulsen F.1, Thale A. 2, Jansen A.1 , Geerling G3, Schaudig U4, Al-Samir K.4, Mentlein R.1, Varoga D.1
1Institute of Anatomy, 2Dept. of Ophthalmology, Christian-Albrechts University, Kiel; 3Dept. of Ophthalmology, Lübeck University, 4Dept. of Ophthalmology, Hamburg University
Purpose: Antimicrobial peptides (AMP) are thought to be effectors of innate immunity through their antibiotic activity and direct killing of microorganism. The induction of AMPs is mediated by activation of human Toll-like-receptors (TLRs) through pathogen-associated microbial products or cytokines. In the present study, we wanted to determine the induction, expression and production of AMP by acinus cells of the lacrimal gland as well as by conjunctival and corneal cells in healthy and pathologic states.
Method: Detection of TLRs 1-5 and several AMP was performed by reverse transcription-polymerase chain reaction (RT-PCR). Deposition of TLRs and AMP was analysed immunohistochemically. Induction experiments were conducted in corneal and conjunctival epithelial cell lines.
Results: RT-PCR revealed TLRs 1-5 in samples of healthy as well as tissues from patients with Non-Sjögren syndrome (NSS) age-related dry eye. Several AMPs were constitutively expressed in the lacrimal gland and at the ocular surface. In addition the inducible AMP human