Programm                 "Degeneration und Regeneration– Grundlagen, Diagnostik und Therapie"

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Computerized Endothelial Cell Density Calculation in the Cornea Bank

Jaroszewski J.1, Rieger R.1, Rieck P.1, Hartmann C.1, Grisan E.2, Ruggeri A.2
1Universitäts-Augenklinik Charité, Campus Virchow Klinikum, Humboldt Universität zu Berlin, Hornhautbank Berlin, 2Università di Padova, Dipartimento di Ingegneria dell'Informazione, Padova

Purpose: We use a computer program (Navis, Nidek Technologies) for the endothelium evaluation of donor corneas using phase contrast microscopy. This software tool offers several functions: cellcount, morphology statistics, density- and area calculation in an automatic, manual and correction mode. We investigated the reliability of the automatic compared to the manual cell count.
Method: 100 corneas with clearly visible cell borders after osmotic treatment in hypotonic BSS were included in this study so far. A 0.1 mm2 frame (ROI) was placed on the computerized endothelial cell image and an automatic (ACC) and manual cell count (MCC) were performed by one experienced investigator. The ACC detects a number of cells and their area size and the MCC uses the ROI size and the number of labelled cells in it for calculating cell density.
Results: In 80% the MCC was higher than the ACC (mean 2318 versus 2190 cells/mm2, mean deviation 128 with a range from 1 to 633 cells/mm2). In 20% the MCC was lower than the ACC (mean 2079 versus 2120 cells/mm2, mean deviation 41 with a range from 1 t

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