Experimental Investigations to the Pathogenesis of Neuronal Degeneration in Cases of Neovascular Glaucoma (NVG)
Thale A., Paulsen F.
Department of Ophthalmology and Institute of Anatomy, Christian-Albrechts University, Kiel
Purpose: The mechanisms of glaucomatous neuronal degeneration are not explained sufficiently. The present study aims at contributing to the understanding of pathogenetic factors for neuronal degeneration in cases of NVG.
Method: 26 enucleated eyes from patients with NVG and 10 adult donor eyes without history of disease were analyzed. Investigations were performed by electron microscopical and histological techniques. Antibodies against different types of collagen, glycosaminoglycans, basal membranes, cytokines and against immune competent cells were used in immunohistochemical reactions.
Results: The characteristic circular arrangement of collagen fibrils in the lamina cribrosa is almost entirely absent in specimens of NVG. The collagen fibrils are arranged in coarse irregular bundles with pronounced differences in caliber. The tight junctions of the endothelial cells within the optic nerve are disconnected from each other at few locations. Immunohistochemical analysis of the lamina cribrosa from patients with NVG reveal that the amount of all examined collagen types and glycosaminoglycans is increased significantly. The number of proliferating cells is distinctly higher. The immunoreactivity of all growth and angiogenesis factors like FGF, TNF alpha and VEGF is stronger in cases of NVG.
Conclusions: A new hypothesis for the pathoge
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