Hotelbuchung
Hotel Registration
Grußwort
Welcome address
Beteiligte Gesellschaften
Societies involved
DOG Information
DOG Information
Eröffnung des Kongresses
Opening Ceremony
Preise
Awards
Ablauf der Tagung 2003
General overview of congress
Lageplan der Räumlichkeiten
Map of Congress Center
Wissenschaftliche Themen
Scientific topics
Symposien
Symposia
Wissenschaftliches Programm
Scientific program
Posterpräsentationen
Poster Presentation
Kurse
Courses
Begleitende Veranstaltungen
Accompanying program
Arbeitssitzungen
Working sessions
Rahmenprogramm
Social program
Allgemeine Informationen
General Information
Autorenindex
Index of Authors
Industrieaussteller
Commercial exhibitors
Sponsoren
Sponsors
Impressum
DOG Homepage
Abstract
Abstract
Comparison between Measurements of Time-resolved Autofluorescence of the Fundus and In-vitro Measurements of Substances
Schweitzer D.1, Hammer M.1, Gaillard E.2
1Experimental Ophthalmology, University Eye Clinic Jena, 2Department of Chemistry and Biochemistry, Northern Illinois University, DeKalb/USA
Purpose: Pathologic alterations of metabolism are discussed as a reason for the development of age-related macular degeneration. For proof of such alterations, the 2-dimensional detection of time-resolved autofluorescence was developed.
Method: It was the goal of the study to compare first in vivo measurements of time-resolved autofluorescence with measurements of isolated substances. Both in vivo and in vitro measurements were performed using the Jena Tau Mapper. In this device, the fluorescence is excited in a 20° field by laser pulses of 100 ps duration at 446 nm. The emission is detected above 475 nm. The fluorescence lifetime was determined in bi-exponential approximation for reduced nicotinamid-adenindinucleotide (NADH), flavinadenindinucleotide (FAD), N-retinyl-N-retinylidene-ethanolamine (A2E), human lipofuscin, and advanced glycation end-product (AGE).
Results: Drawing the results in tau 1 - tau 2 diagrams, substance specific clusters were exhibited. The in vivo measurements result also in clusters, depending on the fundus morphology. An influence of the