|Programm||"Degeneration und Regeneration– Grundlagen, Diagnostik und Therapie"|
Proteomic Patterns of Tear Fluids Obtained by Surface-enhanced Laser Desorption/Ionisation (SELDI) Time-off-flight (TOF) Mass Spectrometry (MS): Potential for New Biomarkers in the Diagnosis of Ocular Diseases
Kariger-Schweigert U. E. M.1, Schweigert F. J.2
Purpose: Qualitative and quantitative determination of tear fluid components is of main interest in ophthalmology. Classical analytical approaches for proteins are 1D-PAGE, immunochemistry or HPLC. More recently proteom analysis involves a tedious and comprehensive high-resolution 2D electrophoresis. The study was conducted to establish high-sensitive high-throughput SELDI-TOF-MS method to characterize tear proteins and peptides.