Programm                 "Degeneration und Regeneration– Grundlagen, Diagnostik und Therapie"


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Abstract
Abstract

Proteomic Patterns of Tear Fluids Obtained by Surface-enhanced Laser Desorption/Ionisation (SELDI) Time-off-flight (TOF) Mass Spectrometry (MS): Potential for New Biomarkers in the Diagnosis of Ocular Diseases

Kariger-Schweigert U. E. M.1, Schweigert F. J.2
1Praxis Drs Kind & Kariger-Schweigert, Falkensee; 2Lehrstuhl für Physiologie und Pathophysiologie, Institut für Ernährungswissenschaft, Universität Potsdam

Purpose: Qualitative and quantitative determination of tear fluid components is of main interest in ophthalmology. Classical analytical approaches for proteins are 1D-PAGE, immunochemistry or HPLC. More recently proteom analysis involves a tedious and comprehensive high-resolution 2D electrophoresis. The study was conducted to establish high-sensitive high-throughput SELDI-TOF-MS method to characterize tear proteins and peptides.
Method: Normal stimulated human reflex tears were collected in capillaries from eight healthy individuals. SELDI-TOF-MS was used for separation and mass characterization of tear proteins. Protein enrichment was performed on a normal phase (NP), a strong cation exchanger (SAX) and a weak anion exchanger (WAX) protein chip surfaces. Subsequently, chip surfaces were washed with buffer to remove non-bound proteins. The molecular mass of the remaining proteins and peptides were determined by TOF-MS.
Results: With the combination of normal phase and ion-exchange chip surfaces up to 30 distinct peaks in the molar range of 4 to 100 kDa can be detected in tear flu


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