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Abstract
Abstract
Degeneration and Regeneration of Normal Conjunctiva - The Role of Apoptosis-associated Genes
Tost F.1, Fabian M.1, Giebel J.2
1Centre of Ophthalmology and 2Institute of Anatomy, Greifswald
Purpose: The balance between cell proliferation and apoptosis is essential to homostasis of the conjunctiva. Apoptosis-associated genes (Bcl-2, Bax, Bad, Caspases) are a causal relationship. Changes of gene expression could be a reason for acut and chronic external diseases through the induction of conjunctival metaplasia. On the other side, the changes of conjunctival epithelium barriere are connected to age-related processes, e.g. loss of goblet cells. The aim of the present study was to evaluate the physiological expression pattern of apoptosis related genes in the conjunctiva.
Method: Furthermore, age-related changes of apoptosis should be differenciated from pathological cellular processes. Therefore, the expression of apoptosis-associated genes in the normal conjunctiva of healthy probands (group A: 27 probands, average mean 22 years and group B: 23 probands, average mean of 69 years ) was evaluated. Epithelial cells were collected from bulbar conjunctiva using modifications of the impression cytology. RNA was isolated from the cytologic impression specimens using a kit. Eluted RNA was used for cDNA-synthesis employing SuperScriptTM II reverse transcriptase according to standard protocol. Different apoptosis genes were detected by PCR amplification using CytoXpressTM Multiplex-PCR with primers for apoptosis-ass