Usefulness of Immunoblotting in the Diagnosis of Human Ocular Toxoplasmosis
Garweg J. G.1, Flückiger F.1, Jacquier P.2
1Dept. of Ophthalmology, University of Bern, Inselspital Bern, Switzerland; 2ParaDiag, Laboratory for Clinical Parasitology, Bern, Switzerland
Purpose: Little only is known about the place of aqueous humour (AH) immunoblotting for the diagosis of ocular toxoplasmosis. Therefore, this prospective study was set up to compare the specific qualitative immune response in aqueous humour and serum of individuals with active ocular toxoplasmosis.
Method: AH and serum from 38 patients with active ocular toxoplasmosis were submitted to immunoblotting for anti-Toxoplasma IgG, IgA, IgM and IgE. The presence of single bands in aqueous humour, but not in serum, bound to antigens with a protein size of 20 - 50 kDA was defined to support a specific local antibody production.
Results: IgG bands were detected in all but 2 cases (95%), IgA bands in 29 instances (76%) respectively. IgM bands were detected sporadically, but always in association with specific IgG, whereas IgE bands were not detected. Specific bands in the aqueous humour, but not in serum, were detected in 24 instances (63%), beyond these 19 of the IgG and 8 of IgA type. In 8 cases (5x IgG, 2x IgA, 1 x both; 21%), the diagnosis was not further supported by laboratory evidence. In 14 instances (37%), no specific bands were detected despite proof of local antibody production by calculation of the C coefficient.
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