Detection and Distribution of Antimicrobial Peptides on the Ocular Surface by Means of Impression Cytology
Aue A.1, Paulsen F. P.2, Müller G.1, Geerling G.1
1Department of Ophthalmology, University Hospital Schleswig-Holstein, Campus Lübeck
2Department of Anatomy, Christian Albrecht University of Kiel
Purpose: Antimicrobial peptids such as human-b-defensin-1 and -2 have been shown to be present on the surface of surgically excised specimen of cornea and conjunctiva. This study aimed to establish an impressionscytological method for the detection and analysis of the geographic distribution of these peptides.
Method: Following topical anaesthesia corneal and conjunctival impression cytologies were obtained by means of celluloseacetate filter membranes. Specimen were collected also bulbar and tarsal conjunctiva in all 4 quadrants of the ocular surface. Immunohistochemistry was performed based on a streptavidin-avidin-peroxidase-reaction.
Results: With this method both, endogenous HBD-1 and inducible HBD-2 were shown to be present on the ocular surface of healthy individuals. Both defensins seemed to be predominantly concentrated around goblet cells.
Conclusions: Antimicrobial peptides could be detected by means of impressionscytology with cellulose-acetate filter membranes and routine immunohistochemistry. This in contrast to previous studies allows the analysis of the geographic distribution of defensins. The accumulation of these peptides around goblet cells
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